Mode of Entry and Sites of Development of Steinernema scapterisci in Mole Crickets.

نویسندگان

  • K B Nguyen
  • G C Smart
چکیده

Infective juvenile steinernematid nematodes have been reported to enter their insect hosts through the mouth, anus, and spiracles (1-3,5-8). Information is lacking, however, for Steinernema scapterisci Nguyen & Smart, 1990 (4), a promising biological control agent of mole crickets in the genus Scapteriscus. This paper reports on the mode of entry of S. scapterisci into mole crickets and the primary sites where they develop. Entry via digestive system: Five mole crickets, Scapteriscus acletus Rehn & Hebard, 1916, were exposed to 8,000 infective juvenile S. scapterisci in a petri dish (90-mm) containing two filter papers (Whatman No. 2). Three hours later, the digestive system was dissected out of each insect and cut into three sections. We found an average of 66 nematodes (range: 62-69) in the anterior section (esophagus to end of crop), none in the middle section (end of crop to junction of Malpighian tubules with gut), and one (0-3) in the posterior section (above junction to anus). In another experiment, mole crickets were inoculated through the mouth or anus with infective juveniles. The beveled tip of a hypodermic needle (B-D Tuberculin, 26G 3/8 , intradermal bevel, Becton and Dickson, Rutherford, NJ) was removed, the end filed smooth, and the syringe was mounted on a stand to facilitate the inoculation pro-

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عنوان ژورنال:
  • Journal of nematology

دوره 23 2  شماره 

صفحات  -

تاریخ انتشار 1991